Klf5 suppresses pERK in mouse ESCs.
- Takuya Azami (2550946)
- Ken Matsumoto (47689)
- Hyojung Jeon (2550952)
- Tsuyoshi Waku (2550955)
- Masafumi Muratani (4760067)
- Hitoshi Niwa (7709)
- Satoru Takahashi (32871)
- Masatsugu Ema (200616)
Publication date
November 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Schematic representation of Klf5 targeting vector to create Klf5-KO ESCs. (B) Expression of Klf5 protein in WT and Klf5-KO ESCs. Scale bar: 100 μm. (C) Western blot analysis of WT, Klf5 heterozygous, and Klf5-KO ESCs. Western blot analysis was performed with antibodies against pERK, ERK, and β-actin. (D) Quantified signal intensity for pERK relative to ERK. Asterisk indicates statistical significance: *P < 0.01; Mann-Whitney U test.</p
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