Additional file 3: of Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling

Figure S3. hUCB-MSC treatment contributes to the recovery of IVH injury-mediated lesions in the hippocampal trisynaptic circuit. (a) Schematic diagram of hippocampal trisynaptic circuit. The perforant path is the connectional route from the entortinal cortex to the dentate gyrus. Signal information from the dentate gyrus projects along the mossy fiber to the cornu ammonis area 3 (CA3). Axons from CA3 project to area CA1 pyramidal neurons via Schaffer collateral fibers. (b) The hippocampal slices of IVH/MSC rat models were cultured at P7. The anterograde axonal tracer biocytin was placed on the entorhinal cortex at DIV8 and fixed with 4% PFA. Biocytin was visualized using the ABC-DAB method. Red arrows indicate the perforant fibers. The square boxed area is enlarged in the bottom panel. Images shown here are representative of at least three independent experiments. Scale bar, 100Â Îźm. (c) Rat hippocampi were dissected and lysed at P7 in IVH/MSCs rat models. Lysates were subjected to IB with the indicated antibodies. Images shown here are representative of at least three independent experiments. (TIF 3251 kb