p35 in the pouch did not interfere with fate change and translocation of the hinge cells.

The embryos were collected at 25°C for 24 h and cultured at 25°C throughout because there was no GAL80ts in these experiments. The larvae were irradiated at 4–5 d after egg laying with 0 or 4000 R of X-rays. -IR samples were dissected 24 h after (mock) irradiation and +IR samples were dissected at times indicated on the figure, fixed and stained for DNA and imaged for RFP/GFP. DNA images were used to locate the pouch when possible (white circles). The discs are shown anterior left and dorsal up when discernable; in irradiated discs, p35 expression results in ‘undead’ cells that overgrow into folded layers (arrowheads), making it difficult to locate the pouch or disc orientation. Scale bar = 100 microns. The genotypes were: (A-B, E-F, I-J) rn-GAL4 control = P{w[+mC] = Ubi-p63E(FRT.STOP)Stinger}9F6/ P{w[+mC] = QUAS-FLPo.P}1; PBac{Disc\RFP[DsRed2.3xP3] = GH146-QF.P}53 P{w[+mC] = QUAS-mtdTomato-3xHA}24A, rn-GAL4/TM6-TB (C-D, G-H, K-L) rn-GAL4>UAS-p35 = P{w[+mC] = Ubi-p63E(FRT.STOP)Stinger}9F6/ P{w[+mC] = QUAS-FLPo.P}1; PBac{Disc\RFP[DsRed2.3xP3] = GH146-QF.P}53 P{w[+mC] = QUAS-mtdTomato-3xHA}24A, rn-GAL4/UAS-p35.