Imiquimod and EAPB0503 trigger an upregulation of TLR-7 and iNOS expression in patients’ derived <i>L</i>. <i>tropica</i> infected macrophages.

(A) Western blot analysis for TLR-7 on patients’ derived L. tropica infected macrophages (from one patient) treated with 0.1 μM of Imiquimod or EAPB0503 for 10 and 24h. (B) RT- PCR detection of iNOS in infected macrophages with patients’ isolated L. tropica amastigotes upon treatment with 0.1 μM of Imiquimod or EAPB0503 for 10 and 24h. Percentage of expression of amastigotes was normalized to GAPDH. Results are expressed as percentage of untreated control (±) SD and are representative of three independent experiments. The t-test was performed to validate significance. *, ** and *** indicate p values ≤ 0.05; 0.01 and 0.001, respectively. P-values less than 0.05 were considered significant.