EAPB0503 exhibits a higher efficacy on freshly isolated <i>L</i>. <i>tropica</i> from patients’ biopsies.

(A) RT- PCR detection of infected macrophages with patients’ derived L. tropica amastigotes treated with 0.1 μM of Imiquimod or EAPB0503 for 10 or 24h, compared to 100 μg/mL of Glucantime alone or combined to 0.1 μM of Imiquimod. Percentage of expression of amastigotes was normalized to GAPDH. Results are expressed as percentage of untreated control (±) SD and are representative of three independent experiments. (B) H&E staining on untreated or treated macrophages infected with patients’ derived L. tropica amastigotes with 0.1 μM of Imiquimod or EAPB0503 for 10 or 24h. The results depict one representative patient. Similar results were obtained on the remaining two patients. (C) Confocal microscopy on patients’ derived L. tropica infected macrophages treated with 0.1 μM of Imiquimod or EAPB0503 for 10 or 24h. The Gp63 surface parasite was stained with an anti-Gp63 antibody (red), and nuclei were stained with Hoechst 33342 (blue). Images represent Z sections. Graphs show quantification of Gp63 (Blind count) as averages of one Z section/cell from 50 different cells of 2 independent experiments on two different patients’ derived amastigotes.