Additional file 1: of Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii

Figure S1. Multiple alignments of amino acid sequences of CS from A. castellanii and other representative organisms. Sequences were aligned using AlignX (Vector NTI 11.5.3, Invitrogen). Sequences are as follows: Ac, A. castellanii AcCS (this study); Bm, (Balamuthia mandrillaris, LEOU01001036); Dd, (Dictyostelium discoideum, XP629379); At (Arabidopsis thaliana, P47998); Eh (E. histolytica, BAA21916); Ei, (Entamoeba invadens, BAN42435); Lm (Leishmania major, CAJ09322); St (Salmonella typhimurium, AGQ86853); Tv (Trichomonas vaginalis, XP001325874). The letter X represents the uncertain sequences of Dictyostelium discoideum CS. Black shading indicates conserved amino acids. Arrowhead indicates active site lysine. Dots indicate binding sites for sulphur incorporation into cysteine. Arrows indicate key OAS-binding sites. Underlined domains indicate β8-β9 residues of CS that interact with SAT in A. thaliana and S. typhimurium. (TIF 6729 kb