Sequence and phylogenetic analyses of the deduced UGT73A17 protein with other closely relative plant UGTs.

(A) Multiple sequence alignment of the PSPG boxes of the deduced UGT73A17 protein with other functionally characterized UGTs at amino acid level. Identical amino acids were indicated in black background, amino acids with 75% and 50% similarities were indicated in dark gray and light gray, respectively. The GenBank accession numbers and plant species of other UGTs are: Scb7GT, BAA83484 (Scutellaria baicalensis); BvGT1, AAS94329 (Beta vulgaris); GeIF7GT, BAC78438 (Glycyrrhiza echinata) and Mt7GT, AAW56091 (Medicago truncatula); (B) Phylogenetic analyses of the deduced UGT73A17 protein with other functionally characterized plant UGTs. Protein sequences were aligned with Clustal W and a neighbor-joining tree was constructed using MEGA 6.0 software. Scale bar indicates the number of amino acid substitutions.