The level of hTERT expression is decreased in AMPKα1 KO cells.

(A) The expression of hTERT mRNA was measured by quantitative real-time PCR in AMPKα1 KO cells. (B) The level of hTERT protein is decreased in AMPKα1 KO cells. Cell lysates were subjected to western blotting with anti-hTERT antibody. (C) The levels of hTERT protein were quantitated. Experiments were performed in triplicate, and the mean and standard deviations are shown in the graph. Control vs. AMPKα1 KO, *P D) The expression of c-Myc protein was downregulated in AMPKα1 KO cells. (E) Schematic representation of AMPK induced activation of hTERT expression.