Intracellular expression of regulatory cytokines in Tregs from cord blood of children of allergic and healthy mothers.

Samples of cord blood of children of healthy (H, n = 112) and allergic (A, n = 98) mothers were stained and analysed by flow cytometry for intracellular presence of cytokines IL-10 and TGF-β. (A) Flow cytometry analysis showing the proportion of IL-10+ cells among CD4+CD25+CD127low Tregs in cord blood (p = 0. 0006). (B) Flow cytometry analysis showing the proportion of TGF-β+ cells among CD4+CD25+CD127low Tregs in cord blood. p values were calculated using the Mann-Whitney test.