Expression of surface markers of Treg function in Tregs from cord blood of children of allergic and healthy mothers.

Samples of cord blood of children of healthy (H, n = 112) and allergic (A, n = 98) mothers were stained and analysed by flow cytometry. (A) Flow cytometry analysis showing the proportion of PD-1+ cells among CD4+CD25+CD127low Tregs in cord blood (p = 0.0382). (B) Flow cytometry analysis showing the proportion of CTLA-4+ cells among CD4+CD25+CD127low Tregs in cord blood. (C) Flow cytometry analysis showing the proportion of GITR+ cells among CD4+CD25+CD127low Tregs in cord blood. p values were calculated using unpaired Student’s t-test.