PC1 activates ERK.

Human CD4+ T cell or PBMC were stimulated for 24 hours with DMSO, kansui, or PC1 at indicated concentrations. Whole cell lystates were separated on 10% SDS PAGE. Membranes were probed with specific antibodies for (A) p-ERK and total ERK (B) CycT1, (C) and p-IκBα and total IκBα, and β-actin. Densitometric data were collected, normalized to appropriate loading controls with the DMSO negative control set to one. Results are representative of western blots from three healthy donors.