Additional file 1: of Hypoxia potentiates gemcitabine-induced stemness in pancreatic cancer cells through AKT/Notch1 signaling

Figure S1. Notch1 inhibition abolishes gemcitabine-enhanced migration, invasion, and chemoresistance. PANC-1 and Patu8988 cells were pretreated with 10 μM DAPT for 24 h and then treated with gemcitabine. (a, b) The transwell migration assay was performed to examine the change in the migratory ability of the cells, and the relative migratory ability was calculated by counting the number of stained cells migrating to the lower chamber. (c, d) The invasive ability of the cells was measured by the transwell invasion assay. (e) After treatment, the MTT assay was performed to test the change in the chemosensitivity of pancreatic cancer cells to gemcitabine. The graphs show the results of three independent experiments. Scale bar, 100 μm. *P < 0.05; **P < 0.01. (TIF 2082 kb