Bacterial load in the lungs, spleen and brain of binge-alcohol mice intranasally infected with different <i>Burkholderia</i> species and doses.

Mice were administered alcohol (4.4g/kg) or PBS (i.p.). At 30 min. post binge alcohol mice were infected with B. thailandensis (3 x 105) (A-B), B. thailandensis (2 x 106) (C-D), or B. vietnamiensis (2 x 106) (E-F). Tissues were collected 24 h (A, D, C) or 72 h (B, D, F) later and bacterial tissue burden was determined (CFU/tissue). Asterisks (*) represent statistical comparisons between alcohol treatment and (Non-Alcohol) control per tissue type determined by one-way ANOVA. Bars represent average CFU (n = 4) with SEM. **, p ≤ 0.01, ***, p ≤ 0.001, ****, p ≤ 0.0001.