Soil humic acids degrade CWD prions and reduce infectivity - Fig 2

(A) HA, extracted from different soils and normalized to 20 g L-1, reduces PrPCWD signal. (B) Quantification of PrP signal (using ImageJ software). Identical amounts of 10% brain homogenate (BHCWD) were incubated overnight at room temperature with water (control), pure HA and HA extracted from Alberta soils: 1 –mountain Brunisol, horizon LF; 2 -northern Luvisol, LFH; 3 –northern Brunisol, LFH; 4—mountain Brunisol, Bm; 5 –northern Luvisol1, Ae; 6 –northern Brunisol, Ae; 7 –central Chernozem1, Ah; 8 –south Chernozem2, Ah. Pure HA was included as a control, (20 g L-1) from two preparations. One solution (first lane, “pure HA”) was prepared directly from the HA powder, and the second (second lane, “pure treat”) solution was treated similar to the HA extraction procedure (Materials and Methods). Samples were analyzed by immunoblotting with Bar224 antibody.