Several miRNAs are upregulated following a blood meal.

Relative miRNA abundance was measured with qRT-PCR. Bars represent the mean ± s.e.m of in 4 independent, biological replicates each containing 3–5 whole body, nondiapausing females. All females were 12 days old and blood fed females ingested a blood meal 36 h before collection. Blood feeding did not significantly change the abundance of miR-124-3p (FDR-adjusted p = 0.102) but did significantly increase the abundance of miR-275-3p, miR-309-5p and miR-375-3p (FDR-adjusted p < 0.05).