D1 vs D2 maps of distant Fos recruitment in limbic structures.

(A) Sagittal maps show relative Fos elevations in each structure induced by NAc laser stimulation in D1 ChR2 mice compared to EYFP control mice (top left), D2 ChR2 mice compared to EYFP controls (top right), and a direct contrast between D1 ChR2 mice and D2 ChR2 mice (bottom left). A baseline comparison map (bottom right) shows elevation of Fos in EYFP mice over unoperated naïve D1/D2 control mice. Both D1 ChR2 and D2 ChR2 stimulations in NAc produced similar anatomical patterns of distant Fos changes in other brain structures. Consequently, and D1 vs D2 ChR2 patterns of distant Fos expression did not significantly differ for any limbic brain structure sampled here. In each map, arrow size denotes effect size of Fos change (assessed using the formula and color of structure denotes percentage change in Fos in that structure. (B) Bar histograms showing mean (+/-SEM) absolute levels of Fos expression for each group/structure. Fos-expression neurons were counted within a 600x800-micron rectangular box sample from each indicated structure or subregion, to standardize sample volume across different structures. Bilateral samples were taken per mouse (one per hemisphere) and averaged for a single Fos count. Red bars = D1 ChR2 mice; Blue = D2 ChR2 mice; Yellow = EYFP mice; gray = unoperated/surgically-naïve control mice. * = different from EYFP control at p = 0.05. # = different from surgery naïve controls at p = 0.05. Brain region abbreviations are: Prelimbic cortex (PrL), Infralimbic cortex (IL), Basolateral Amygdala (BLA), central nucleus of Amygdala (CeA), ventral subiculum (vSub), rostral NAc Shell (rShell), rostral NAc core (rCore), caudal half of NAc medial shell (cShell), caudal NAc Core (cCore), rostral ventral pallidum (rVP), caudal Ventral pallidum (cVP), lateral hypothalamus (LH), ventral tegmental area (VTA), substantia nigra (SN).