Methacholine-induced airway constriction in mouse, rat, marmoset, and human PCLS compared with pre-incubation with IL-13 of the corresponding species (except for marmoset, rhIL-13 was used).

Macroscopic images of precision-cut lung slices of untreated PCLS (control) or PCLS treated with 8 nM of IL-13 for six hours prior to stimulation with 10−10 M and 10−3 M methacholine (MCh), the lowest and the highest concentration, respectively. Scale bar: 500 μm.