Figure S1. KRAS activation by evaluating the downstream protein of RAS cascade. ID8-GFP and ID8-KRAS-GFP cells (1 à 106) were incubated for 48 hours. Attached cells were collected, washed with PBS, and proteins were extracted from cells. Cell lysates were subjected to Western blotting analysis for expression of phospho-MEK1/2, MEK1/2, phospho-ERK1/2, ERK1/2 and anti-β-actin. (PDF 68 kb
Figure S8. Schematic representation of the involvement of NRAS in MEK/ERK signaling pathway. (PDF 12...
Combined knockdown of MEK1 and MEK2 causes prolonged Ras activation. EGF-induced Ras activation was ...
Figure S2 4EBP1 knockdown slows proliferation of SUM-229 and SUM-149 breast cancer cells. (a) Wester...
Table S1. KEGG pathway analysis for genes upregulated in ID8-KRAS-3D cells compared to ID8-3D cells....
Figure S2. GFP-positive cancer cells in ID8 and ID8-KRAS cells in vivo. Mice were i.p. injected with...
Figure S3. Assessment of apoptosis in ID8 and ID8-KRAS cells in vitro and in vivo. a ID8 and ID8-KRA...
Supplementary methods. Methods of western blot analysis, in vitro apoptosis assessment using Annexi...
Supplementary File 1. All the cDNA microarray data for analysis of ID8-2D, ID8-3D, ID8-KRAS-2D, and...
Figure S1. Genome-wide synthetic lethal screens with RAS1(V19) and RAS2(V19) identify overlapping se...
Figure S1. Upper: The fluorescence microscopy detects the transfection efficiency of each shRNA (KD1...
contains Tables S1-S9. Table S1. Results of the primary yeast screen with RAS alleles RAS1 and RAS2 ...
Figure S6. Silencing of mutant KRAS reduced sensitivity of cells to KRA-533. (A) KRAS shRNA plasmids...
Figure S1. Protein activity suppressed by inhibitors. Western blot quantification and short time poi...
Figure S2. KRA-533 induces caspase 3 activation and reduces mitochondrial membrane potential in NSCL...
Table S2. The time-dependence of RMSD and RG by D14 and C22 compounds that target wild-type and muta...
Figure S8. Schematic representation of the involvement of NRAS in MEK/ERK signaling pathway. (PDF 12...
Combined knockdown of MEK1 and MEK2 causes prolonged Ras activation. EGF-induced Ras activation was ...
Figure S2 4EBP1 knockdown slows proliferation of SUM-229 and SUM-149 breast cancer cells. (a) Wester...
Table S1. KEGG pathway analysis for genes upregulated in ID8-KRAS-3D cells compared to ID8-3D cells....
Figure S2. GFP-positive cancer cells in ID8 and ID8-KRAS cells in vivo. Mice were i.p. injected with...
Figure S3. Assessment of apoptosis in ID8 and ID8-KRAS cells in vitro and in vivo. a ID8 and ID8-KRA...
Supplementary methods. Methods of western blot analysis, in vitro apoptosis assessment using Annexi...
Supplementary File 1. All the cDNA microarray data for analysis of ID8-2D, ID8-3D, ID8-KRAS-2D, and...
Figure S1. Genome-wide synthetic lethal screens with RAS1(V19) and RAS2(V19) identify overlapping se...
Figure S1. Upper: The fluorescence microscopy detects the transfection efficiency of each shRNA (KD1...
contains Tables S1-S9. Table S1. Results of the primary yeast screen with RAS alleles RAS1 and RAS2 ...
Figure S6. Silencing of mutant KRAS reduced sensitivity of cells to KRA-533. (A) KRAS shRNA plasmids...
Figure S1. Protein activity suppressed by inhibitors. Western blot quantification and short time poi...
Figure S2. KRA-533 induces caspase 3 activation and reduces mitochondrial membrane potential in NSCL...
Table S2. The time-dependence of RMSD and RG by D14 and C22 compounds that target wild-type and muta...
Figure S8. Schematic representation of the involvement of NRAS in MEK/ERK signaling pathway. (PDF 12...
Combined knockdown of MEK1 and MEK2 causes prolonged Ras activation. EGF-induced Ras activation was ...
Figure S2 4EBP1 knockdown slows proliferation of SUM-229 and SUM-149 breast cancer cells. (a) Wester...