Classification of quiescent cells and proliferating cells recovered from quiescence.

PLS-LDA of Raman (A, B) and FT-IR spectra (C, D) of contact-inhibited quiescent cells and proliferating cells recovered from quiescence after 14 and 100 days. Distribution of cells shown in one-dimensional scatter plots combined with box-plots (A, C) showing 95% confidence interval with p-values <0.001 for Raman and FT-IR data. Cultivation time was specified by color (14 days: gray, 100 days: black). Mean and difference spectra (“diff”, proliferating cells recovered from quiescence vs. quiescent cells) from the classified cells are plotted together with the PLS-LD coefficients vs. the wavenumber (B, D).