Additional file 1: of Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response
- Zhifeng Chen (2340808)
- Kara Cox (6039371)
- Aimin Tang (2817031)
- Jeanette Roman (6039374)
- Malorie Fink (6039377)
- Robin Kaufhold (6039380)
- Liming Guan (2578543)
- Andy Xie (6039383)
- Melissa Boddicker (6039386)
- Debra Mcguinness (6039389)
- Xiao Xiao (99147)
- Hualin Li (438300)
- Julie Skinner (3709885)
- Thorsten Verch (6039392)
- Mary Retzlaff (6039395)
- Kalpit Vora (6039398)
Publication date
December 2018
DOIAbstract
Figure S1. FACS sorting for single plasmablast cells. Freshly harvested peripheral blood mononuclear cells were stained with antibodies for flow cytometric detection of plasmablast cells. Plasmablasts were gated as shown, defined as lymphocytes/single cells/CD3-/CD19+ and low/CD27hiCD38hi/CD20 low, and sorted into single wells of a 96 well plate for RNA preservation and cloning. (PDF 1246âkb
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