Table S9. Plasmids used in this study (including sequences of synthetic oligonucleotides that were used for sgRNA guide sequence cloning), and primer sequences used for analysis of editing at the corresponding genomic sites by T7E1 and TIDE analyses). (XLSX 30 kb
Table S1. The frequencies of accurate NHEJ in repair of Cas9-induced DSBs. Table S2. Top five group ...
Figure S1. Genome-wide computational prediction of NmeCas9 off-target sites using CRISPRseek with th...
Unique and multiple repeated sequences for targeting by a single specific sgRNA. (XLSX 9524 kb
Table S5. Targeted deep sequencing read count data for all sgRNAs used and for all off-targets detec...
Table S4. Summary of potential off-target sites detected by SITE-seq analysis. The table includes si...
Table S2. Summary of potential off-target sites detected by GUIDE-seq analysis using the Bioconducto...
Table S3. Targeted deep sequencing read count data for all sgRNAs used and for all off-targets detec...
Table S1. List of off-target sites with canonical and variant SpyCas9 and NmeCas9 PAMs predicted by ...
Figure S1. (A) Sequences of sgRNAs associated with their respective DNA targets used in Fig. 1b. (B...
Complete DNA sequences of the sgRNA expression constructs. Figure S2. Comparison of the on-target ac...
Figure S1. Full plasmid sequences of the Cas9, gRNA, and donor vectors. Green, orange, blue, red, li...
Additional file 1: Fig. S1. Flow diagram for designing and assessing inlaid-Nme2ABEs. Fig. S2. Compa...
Table S2. Sequences of targeting crRNAs used for in vitro RNA transcription. Table S3. Target sequen...
Background: The development of CRISPR genome editing has transformed biomedical research. Most appli...
Figure S6. UDiTaS characterization and comparison to AMP-Seq with plasmid standards. Plasmids contai...
Table S1. The frequencies of accurate NHEJ in repair of Cas9-induced DSBs. Table S2. Top five group ...
Figure S1. Genome-wide computational prediction of NmeCas9 off-target sites using CRISPRseek with th...
Unique and multiple repeated sequences for targeting by a single specific sgRNA. (XLSX 9524 kb
Table S5. Targeted deep sequencing read count data for all sgRNAs used and for all off-targets detec...
Table S4. Summary of potential off-target sites detected by SITE-seq analysis. The table includes si...
Table S2. Summary of potential off-target sites detected by GUIDE-seq analysis using the Bioconducto...
Table S3. Targeted deep sequencing read count data for all sgRNAs used and for all off-targets detec...
Table S1. List of off-target sites with canonical and variant SpyCas9 and NmeCas9 PAMs predicted by ...
Figure S1. (A) Sequences of sgRNAs associated with their respective DNA targets used in Fig. 1b. (B...
Complete DNA sequences of the sgRNA expression constructs. Figure S2. Comparison of the on-target ac...
Figure S1. Full plasmid sequences of the Cas9, gRNA, and donor vectors. Green, orange, blue, red, li...
Additional file 1: Fig. S1. Flow diagram for designing and assessing inlaid-Nme2ABEs. Fig. S2. Compa...
Table S2. Sequences of targeting crRNAs used for in vitro RNA transcription. Table S3. Target sequen...
Background: The development of CRISPR genome editing has transformed biomedical research. Most appli...
Figure S6. UDiTaS characterization and comparison to AMP-Seq with plasmid standards. Plasmids contai...
Table S1. The frequencies of accurate NHEJ in repair of Cas9-induced DSBs. Table S2. Top five group ...
Figure S1. Genome-wide computational prediction of NmeCas9 off-target sites using CRISPRseek with th...
Unique and multiple repeated sequences for targeting by a single specific sgRNA. (XLSX 9524 kb