Expression of flowering repression genes <i>MAF5</i>, <i>MAF4</i> and <i>FLC</i> were higher in it:<i>cbf123</i> plants than in IT plants.

Expression levels of MAF4, MAF5, and FLC were determined either by RNAseq (A) or by quantitative RT-PCR (B) in IT and it:cbf123 plants grown at 22°C (warm) or at 22°C followed by 2 weeks at 4°C (2wkV) under a 16-h photoperiod. Error bars indicate SE of two (A) or three (B) biological replicates. FPKM = Fragments Per Kilobase of transcript per Million mapped reads. Asterisks indicate a significant difference in expression level between IT and it:cbf123 plants (Student t-test, * indicates P<0.05, ** indicates P<0.01).