Targeted CovS inactivation recapitulates the M4-LC-1 phenotype.
- Jessica Galloway-Peña (186182)
- Sruti DebRoy (159313)
- Chelcy Brumlow (5917472)
- Xiqi Li (6054014)
- Truc T. Tran (310042)
- Nicola Horstmann (201711)
- Hui Yao (240607)
- Ken Chen (61463)
- Fang Wang (105926)
- Bih-Fang Pan (4255138)
- David H. Hawke (460602)
- Erika J. Thompson (6054017)
- Cesar A. Arias (310046)
- Vance G. Fowler Jr. (223373)
- Micah M. Bhatti (6054020)
- Awdhesh Kalia (78115)
- Anthony R. Flores (405428)
- Samuel A. Shelburne (251582)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Targeted inactivation of covS in strain M4-SC-1 produced a large colony phenotype analogous to that seen in strain M4-LC-1 (see Fig 1A for comparison). (B) Select gene transcript levels in the naturally occurring (M4-LC-1) and isogenic (M4-SC-1ΔcovS) CovS mutants compared to the wild type CovS intact strain M4-SC-1. Data graphed are mean ± standard deviation of two biological replicates, with two technical replicates, done on two separate days (n = 8). (C-E) Transmission electronic microscopy at 40,000x magnification of indicated strains showing that targeted inactivation of covS reproduces the rough surface phenotype (indicated by arrows) seen in strain M4-LC-1.</p
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