MOESM4 of EZH2 variants differentially regulate polycomb repressive complex 2 in histone methylation and cell differentiation

Additional file 4: Fig. S4. EZH variants interact with other PRC2 subunits. (a) The interaction between EZH2 and EED or SUZ12 in ES cells and spermatocytes was examined by coimmunoprecipitation assays. (b, c) The interaction between EZH variants and PRC2’s accessory subunits was examined by coimmunoprecipitation assays. HEK293T cells were cotransfected with each of EZH variants and of accessory subunits and harvested for assays two days post-transfection. (d) Comparison of EZH2 protein levels between ES cells and spermatocytes by Western blot analysis. Nucleolin serves as a control