<i>In vivo</i> effects of 2HF and meglumine antimoniate using wild-type <i>L</i>. <i>amazonensis</i>.

BALB/c mice were infected in the right ear with 2 × 106 wild-type L. amazonensis promastigotes. Panel A: Lesion development on the animals treated orally with 2HF (50 mg/kg/day), intraperitoneally with meglumine antimoniate (100 mg/kg/day) and with an oral suspension added to DMSO (0.2% v/v) (2HF vehicle). The treatment started seven days post-infection and was given once daily seven times per week until the end of the experiment (day 42). Panel B: Parasite burden of the L. amazonensis-infected BALB/c mice untreated or treated with 2HF (50 mg/kg/day) or meglumine antimoniate (100 mg/kg/day). Ear parasite loads were determined via a limiting dilution assay. Data are expressed as the means ± standard errors. These data represent two independent experiments with five mice per group each (n = 5). *, ** and *** indicate significant differences relative to the control group and #, ##, ### indicate significant differences relative to 2HF (p < 0.05; p< 0.01 and p < 0.001, respectively); Panel C: Illustrative lesion photos of a representative infected ear treated with the vehicle (left photo), 2HF (center photo) and meglumine antimoniate (right photo). 2HF = 2’-Hydroxyflavanone.