Gene signature dissimilarity among four groups with different PD-L1 and B-cell infiltration levels.

(A) Silhouette score with different numbers of clusters, calculated using cluster robustness analyses. (B) Heat map with the top 100 differentially expressed genes (DEGs) in the four groups. DEGs with a significant false discovery rate were analyzed using ANOVA-like tests, after which unsupervised hierarchical cluster analysis was performed. Stable clusters I and II were generated. The pie chart shows distributions of patients (%) from the four groups in clusters I (C) and II (D).