Study procedures.

(A): Posterior view of porcine larynx hemisected through the posterior cricoid cartilage and held open with a 15-mL plastic centrifuge tube. Asterisk: arytenoid complex. Arrowheads: membranous vocal folds. (B): Timeline of study procedures. 30 porcine larynges, 5 per group, underwent PD-MRI scans at baseline and after immersion in water (H2O), phosphate-buffered saline (PBS), 5%, 10%, or 30% NaCl, or dry air. Larynges dehydrated in NaCl solutions or dry air were rehydrated in H2O and scanned a third time. (C): Exemplar selections for intensity measurement in a vocal fold slice. Asterisks: arytenoid complex. Yellow circles (D = 0.8–0.9 mm): intensity in anterior, middle, and posterior thirds of membranous vocal fold mucosa. Green circles (D = 0.8–0.9 mm): intensity in anterior, middle, and posterior thirds of thyroarytenoid muscle. Red circle (D = 1.5 cm): intensity of phantom.