Additional file 1: of Microbial dysbiosis and mortality during mechanical ventilation: a prospective observational study

Supplementary methodology and results. Table S1. ICU samples collected and additional information concerning the patients included in the study. Table S2. Samples collected from healthy donors. Table S3. OTUs significantly different in the lower respiratory tract between healthy donors and ICU patients. Table S4. OTUs that are significantly different in stool between healthy donors and ICU patients. Table S5. Correlation matrix results using Spearman rank coefficient correlation between metadata and α-diversity metrics of ETAs. Figure S1. Greater heterogeneity within anatomical site in the ICU cohort in comparison to a healthy cohort. Figure S2. Gastric microbial diversity is not associated with illness severity in critical ill patients. Figure S3. Microbial profiles of the ETA specimens collected from critically ill patients. Figure S4. Lack of association between hospital mortality and bacterial load in lower respiratory tract samples. Figure S5. Absence of detectable difference within microbial diversity between categories of admission. Figure S6. Antimicrobials exposure is not associated with ETA microbial diversity. Figure S7. No association between ICU samples microbial diversity and hospital mortality using the Observed Species index. Figure S8. Inflammatory markers and APACHE II score are not statistically different between deceased and discharged alive patients. Figure S9. OTU5 does not influence the loss of biogeographical distinction in ICU patients. Figure S10. APACHE II score association with α-diversity remains when OTU5 is removed from the analysis. Figure S11. OTU5 removal does not impact the decrease within microbial diversity observed in the deceased group for ETAs. Figure S12. Association between ICU samples microbial diversity and patient’s outcomes when OTU5 is removed. (DOCX 2905 kb