A comparison of selected CSF miRNA levels in RNA samples extracted by four RNA isolation kits with various protocol modifications, including different volumes of CSF input (1 ml and 0.5 ml) and adding (+) or omitting of glycogen during extraction.

Levels of cel-miR-39 (A), miR-10a (B), and miR-196a (C) were analyzed using Real-Time PCR in RNA samples extracted from two GBM and two non-tumor CSF pools. Levels of miR-16 (D) were analyzed in paired RNA samples extracted from six independent CSF samples.