STAT6 knockdown significantly turns over cellular gene expression of PEL cells.

(A) The expression of 49 cellular genes was significantly affected by STAT6 knockdown in PEL cells. BC3 and BCBL1 cells with or without STAT6 knockdown (S6 Fig) were individually subjected to RNA deep-sequencing analysis. The cellular genes with significantly change in the presence of STAT6 knockdown are shown. (B) Reduction of STAT6 led to the increased gene expression of MHC II-, cytokine- or anti-apoptosis-related proteins in PEL cells. The molecules related to antigen processing and presentation, cell migration, tumor suppressor, and chromosome stability are highlighted. (C) Expression of TRIML2 and AIM1 was consistently enhanced by RTA or STAT6 knockdown. The results were the RNA deep-sequencing analysis of iSLK cells with Doxycycline-inducible RTA (S5 Fig) and PEL cells with STAT6 knockdown. (D) Quantitative PCR analysis of TRIML2, AIM1 and CIITA expression in the iSLK-RTA and iSLK-219 cells treated with Doxycycline, or BCBL1 cells treated with TPA and sodium butyrate (T/NB) for 24 h.