Additional file 3: of The isomiR-140-3p-regulated mevalonic acid pathway as a potential target for prevention of triple negative breast cancer

Figure S2. Cholesterol pathway mediators HMGCR and HMGCS1 increase during breast cancer progression. (A) Filters to integrate miR-140-3p-1 expression with RNA-seq results of MCF10A breast cancer progression panel to identify functional gene targets of miR-140-3p-1. (B) Top deregulated pathways during breast cancer progression identified using ingenuity pathway analysis. The mevalonate pathway was identified as the top pathway. (C and D) Endogenous HMGCR and HMGCS1 mRNA levels in a MCF10A-based breast cancer progression model. Levels were determined by qPCR. Values are normalized toRPL19 mRNA levels and represent mean fold change (± SEM) relative to MCF10A(P): *p < 0.05. (PPTX 59 kb