MOESM1 of Dexmedetomidine expands monocytic myeloid-derived suppressor cells and promotes tumour metastasis after lung cancer surgery

Additional file 1: Figure S1. Expansion of M-MDSC by dexmedetomidine is inhibited by yohimbine. (A, B) CD11b+CD33+HLA-DR− cells (3 × 104 cells/well) isolated from lung cancer patients (n = 6) 24 h after surgery were cocultured with (A) dexmedetomidine (DEX) or (B) dexmedetomidine and yohimbine (DEX + YOH). Twenty-four hours after coculture, floating cells were collected and numerated using an automated cell counter. The percentage of CD11b+CD33+HLA-DR−CD14+ (M-MDSC) was analyzed by flow cytometry and the absolute number of M-MDSC was calculated