Single cell dissection reveals subsets within the potentially CD8 acceptor B cell pool.
- Ulrike Strittmatter-Keller (6078497)
- Caroline Walter (6078500)
- Celine Rauld (1697938)
- Nicole Egli (824646)
- Camille Regairaz (6078503)
- Sabine Rabe (6078506)
- Gerhard Zenke (2342968)
- José Carballido (6078509)
- Tamás Schweighoffer (6078512)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) IL6R (CD126) is expressed on a small subset of CD19+ cells (right panel), that is only a fraction of the corresponding total CD19+CD27+CD200- pool (left panel). Numbers indicate the percentage of gated cells within all B lymphocytes. (B) Two-dimensional t-SNE analysis of all single cell events (n = 231) pooled from two apheresis and two PBMC donors; each symbol represents an individual cell. Subsets are numbered and color coded according to their assignment by R-PhenoGraph. Ellipses represent the 95% confidence interval for each color matched population. (C) The single cell–based subsets resolved according to their phenotypic (FACSort) origin, using the same t-SNE and R-PhenoGraph annotation as in (B).</p
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