CD8 positive B cells release cytokines and immunoglobulins.

(A) Production of the cytokines EBI3 and IL-35 was determined by ELISA from supernatants of freshly sorted CD8+ B cells (black bars) and CD8- control B cells (white bars). Cultures contained 50’000 cells in 200 μl volume. As indicated, samples for both measurements were obtained at 18h and 4 days, without or with CD40+IL-4 stimulation. The dashed line indicates the assay detection limit. (B) Production of IgG was determined by ELISPOT using cells sorted either from apheresis material or from peripheral blood (PBMC). Spots were counted, and are expressed as percentage of input cells (3’000 or 1’000 cells per well, as indicated). Cultures were performed without (black bars) or with CD40L+IL-4 stimulation (white bars).