The areas containing the cofilin spots (I to IV) have been selected for the sake of the clarity of the figure. The protein separation used the same pH gradient as the one used in Fig 1. Note the close vicinity of other protein spots (X) with the phosphocofilin spot (III) in the murine cell lines. In these images, proteins were detected by silver staining.</p
<p>(A) Immunolocalization of phosphorylated cofilin (P-cofilin, green) and F-actin phalloidin staini...
<p>A. F-actin was stained with phalloin conjugated to a fluorophore (red) in HCT116 and HCTshEZ-2 ce...
<p>After the first dimension the IPG strip was applied to the top of a precast Criterion XT Bis-Tris...
The process of alignment and detection of cofilin and phosphocofilin spots has been carried out on t...
The data for spot I abundance are expressed in percentage of the sum of all spots measured on the si...
To reduce the spot crowding around the phosphocofilin spot, a linear 4–8 pH gradient was used. This ...
<p>Cells were lysed and the amount of phosphorylated Cofilin and of total Cofilin present in the lys...
<p>(<b><i>A</i></b>) The levels of total and phosphorylated cofilin proteins in Du145-Mock and -KAI1...
<p>(A) Distribution of vinculin (red), actin (blue), and cofilin (green) in OCs differentiated on gl...
<p>A. HCT116, RKO, HCT-shEZ-2 and HT29 have been co-stained with p-cofilin (green) and G-actin (red)...
<p>Immunostaining for total cofilin-1 and p-cofilin in FFPE sections of (A and B, respectively) surg...
The phosphosites are displayed on the sequence of human cofilin. star: already known and described i...
<p>Two-dimensional electrophoregram of condition media from (A) BPH-1, (B) LNCaP, and (C) C4-2 cells...
<p>BMMs were cultured for 3 days in the absence (−) or in the presence (d1 to d3) of RANKL. Each day...
<p>BMMs were infected with viral supernatants encoding GFP-tagged Cofilin-WT, -S3A and -S3D, then di...
<p>(A) Immunolocalization of phosphorylated cofilin (P-cofilin, green) and F-actin phalloidin staini...
<p>A. F-actin was stained with phalloin conjugated to a fluorophore (red) in HCT116 and HCTshEZ-2 ce...
<p>After the first dimension the IPG strip was applied to the top of a precast Criterion XT Bis-Tris...
The process of alignment and detection of cofilin and phosphocofilin spots has been carried out on t...
The data for spot I abundance are expressed in percentage of the sum of all spots measured on the si...
To reduce the spot crowding around the phosphocofilin spot, a linear 4–8 pH gradient was used. This ...
<p>Cells were lysed and the amount of phosphorylated Cofilin and of total Cofilin present in the lys...
<p>(<b><i>A</i></b>) The levels of total and phosphorylated cofilin proteins in Du145-Mock and -KAI1...
<p>(A) Distribution of vinculin (red), actin (blue), and cofilin (green) in OCs differentiated on gl...
<p>A. HCT116, RKO, HCT-shEZ-2 and HT29 have been co-stained with p-cofilin (green) and G-actin (red)...
<p>Immunostaining for total cofilin-1 and p-cofilin in FFPE sections of (A and B, respectively) surg...
The phosphosites are displayed on the sequence of human cofilin. star: already known and described i...
<p>Two-dimensional electrophoregram of condition media from (A) BPH-1, (B) LNCaP, and (C) C4-2 cells...
<p>BMMs were cultured for 3 days in the absence (−) or in the presence (d1 to d3) of RANKL. Each day...
<p>BMMs were infected with viral supernatants encoding GFP-tagged Cofilin-WT, -S3A and -S3D, then di...
<p>(A) Immunolocalization of phosphorylated cofilin (P-cofilin, green) and F-actin phalloidin staini...
<p>A. F-actin was stained with phalloin conjugated to a fluorophore (red) in HCT116 and HCTshEZ-2 ce...
<p>After the first dimension the IPG strip was applied to the top of a precast Criterion XT Bis-Tris...