P53 participates in the sensitization effect of GSK-3 inhibitors on TRAIL-induced apoptosis.

(A) AGS were incubated with or without TRAIL (25 ng/mL), SB-415286 (25 μM) and/or LiCl (20 mM) for 12 hours. Nuclear extracts and whole cell were analyzedfor p53 expression by Western blot. AGS were transfected with p53 or irrelevant siRNA and treated with or without TRAIL (25 ng/mL) and LiCl (20 mM) for 24 hours and then evaluated for p53 expression by Western blot (B) and colorimetric determination of caspase-3 activity (C). Representative blots of four independent experiments are shown. The results are shown as the mean ±the standard error (SE) of four independent experiments. *, p < 0.05 when compared to cells transfected with irrelevant siRNA.