Immunohistochemical analyses of antigen-presenting cells in the oral mucosa of Ni-induced allergic mice.

Frozen buccal mucosa sections were stained with anti-F4/80 antibodies at days 1, 3, and 7 after challenge. At day 1 after challenge, abundant infiltration of macrophages into the epithelial basal layer and the upper dermis was observed in ACM mice (G). Scale bar = 10 μm.