In-Taken Labeling and in Vivo Tracing Foodborne Probiotics via DNA-Encapsulated Persistent Luminescence Nanoprobe Assisted Autofluorescence-Free Bioimaging

An in vivo probing strategy that can real-time and in situ trace target probiotics inside the living body is herein proposed by employing plasmid-like DNA as in-taken assistance, persistent luminescence nanophosphors (PLNPs) as optical labeling, and background-free fluorescence bioimaging as signal readout. PLNPs with superlong afterglow and excellent biocompatibility and stability were surface-modified by DNA molecules with a specific sequence, which greatly promoted the nanoparticle penetration into the bacteria and facilitated the in vivo bioimaging with high sensitivity and signal-to-noise ratio. Compared with the previous surface-labeling strategy by antibody recognition, the in-taken optical labeling demonstrated improved stability, and reached ideal results of real-time and in situ monitoring the in vivo behaviors of target probiotics, supporting the further development of in vivo investigation methodology for foodborne probiotics. Moreover, such a strategy offers a promising platform that leverage nanoscience to food nutrition as well as food-safety research, aiming to collect more accurate and fresh information from the living body