Analysis of DNA extracted from Formalin-Fixed Paraffin-Embedded (FFPE) clinical samples.

The spotting schema of the barcode sequences is the same of Fig 2A. (A) Cy3 fluorescence image and the plot of the relative fluorescence intensity of the sample identified with KRAS G12S mutation. (B) Cy3 fluorescence image and the plot of the relative fluorescence intensity of the sample identified with KRAS Q61H1 (c.183A>C) mutation. (C) Cy3 fluorescence image and the plot of the relative fluorescence intensity of the sample identified with NRAS G12V mutation. The yellow squares in the images were used to highlight more easily the analyzed spots. The bars are the average of the intensity of the 4 spots (2 X 2 subarray) of each barcode probe subarrays. The error bars are the standard deviations of the fluorescence intensity of each sample. Q61H1 c.183A>C, Q61H2 c.183A>T.