Additional file 4: of Analysis of spinal and muscle pathology in transgenic mice overexpressing wild-type and ALS-linked mutant MATR3

Figure S2. MATR3 is not robustly elevated in the spinal cords of MATR3WT and MATR3F115C mice. a Northern blot analysis of cervical spinal cord showed limited transgenic MATR3 RNA expression in MATR3WT (lead line 1563 and validation line 1554) and MATR3F115C (lead line 1576) mice at ~ 4 months of age. Mouse prion (PrP) mRNA served as a loading control. b Western blot demonstrated that total MATR3 is not robustly elevated in spinal cord of MATR3F115C (validation line 1579) mice compared to NT mice at ~ 1.5 months of age. GAPDH was utilized as a loading control. c Quantification of Western blot showed no significant difference in total MATR3 levels in spinal cord of NT compared to MATR3F115C validation line 1579 mice (t-test, p > 0.05). (TIF 907 kb