Characterization of the humoral responses induced in immunized rabbits.
- Carolina Beltran-Pavez (6103037)
- Carolina B. Ferreira (6103040)
- Alberto Merino-Mansilla (781674)
- Amanda Fabra-Garcia (6103043)
- Maria Casadella (6103046)
- Marc Noguera-Julian (440725)
- Roger Paredes (180297)
- Alex Olvera (3343655)
- Isabel Haro (2148178)
- Christian Brander (64220)
- Felipe Garcia (5660647)
- Jose M. Gatell (5657728)
- Eloisa Yuste (5664688)
- Victor Sanchez-Merino (5653459)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
The humoral immune response of the immunized rabbits was characterized at week 15. (A) The presence of anti-HIV antibodies in rabbit sera were analyzed by western blot. Preimmune sera of rabbit 1640 was also included as an additional negative control. Endpoint ELISA titters to autologous (AC10.29; B) and heterologous (Bal; C) rgp120. Open symbols correspond to animals immunized with no adjuvant. The highest dilution which gave an OD450 2.5 folds higher than pre-immune sera without dilution was designated as the antibody endpoint titter. Results showed data of three independent experiments and results were expressed as the mean ±SD (n = 4). Ns:non significant; *p<0.05 Mann-Whitney U test.</p
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