Additional file 11: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Additional file 7: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S2. Likelihood ratio test for dominance of fertilization types in nodes. (XLSX 8 kb

Additional file 10: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S3. Results from phylogenetic generalized least squares (PGLS) analyses of semen diffusion and...

Additional file 5: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S1. Distinct fertilization types in Lake Tanganyikan cichlids and ancestral reconstruction of...

Additional file 8: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S2. Evolutionary transition of fertilization types by restriction of BayesTraits. (PDF 685 kb

Additional file 13: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S5. Evolutionary transition of oral fertilization and glycosylation of SPP120. (PDF 770 kb

Additional file 28: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S9. Results from phylogenetic generalized least squares (PGLS) analyses of SPP expression and ...

Additional file 26: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S8. Test for accelerated evolution in different fertilization manners in the groups. (DOCX 12 ...

Additional file 22: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S5. Results from phylogenetic generalized least squares (PGLS) analyses of sperm immobilizatio...

Additional file 3: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movie S3. Spawning behavior of the stocked-spermatozoa fertilization cichlid Cyathopharynx furcifer....

Additional file 20: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S7. Far-Western analyses of SPP120 on the Triton-soluble fraction (Tx) or remnant fraction of...

Additional file 21: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S8. Immobilization/aggregation effect of SPP120 from various species. (PDF 421 kb

Additional file 15: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movie S6. Sperm aggregation in the presence of full length SPP120 (FullSPP). (MOV 403 kb

Additional file 23: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S9. Phylogenetic tree, motif structure of SPP120s and their molecular sequence evolution. (EP...

Additional file 6: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S4. Results from phylogenetic generalized least squares (PGLS) analyses of semen diffusion and...

Additional file 2: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movies S2. Spawning behavior of the oral fertilization cichlid Ophtalmotilapia ventralis. (MOV 1450 ...

Additional file 7: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S2. Likelihood ratio test for dominance of fertilization types in nodes. (XLSX 8 kb

Additional file 10: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S3. Results from phylogenetic generalized least squares (PGLS) analyses of semen diffusion and...

Additional file 5: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S1. Distinct fertilization types in Lake Tanganyikan cichlids and ancestral reconstruction of...

Additional file 8: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S2. Evolutionary transition of fertilization types by restriction of BayesTraits. (PDF 685 kb

Additional file 13: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S5. Evolutionary transition of oral fertilization and glycosylation of SPP120. (PDF 770 kb

Additional file 28: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S9. Results from phylogenetic generalized least squares (PGLS) analyses of SPP expression and ...

Additional file 26: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S8. Test for accelerated evolution in different fertilization manners in the groups. (DOCX 12 ...

Additional file 22: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S5. Results from phylogenetic generalized least squares (PGLS) analyses of sperm immobilizatio...

Additional file 3: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movie S3. Spawning behavior of the stocked-spermatozoa fertilization cichlid Cyathopharynx furcifer....

Additional file 20: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S7. Far-Western analyses of SPP120 on the Triton-soluble fraction (Tx) or remnant fraction of...

Additional file 21: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S8. Immobilization/aggregation effect of SPP120 from various species. (PDF 421 kb

Additional file 15: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movie S6. Sperm aggregation in the presence of full length SPP120 (FullSPP). (MOV 403 kb

Additional file 23: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S9. Phylogenetic tree, motif structure of SPP120s and their molecular sequence evolution. (EP...

Additional file 6: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S4. Results from phylogenetic generalized least squares (PGLS) analyses of semen diffusion and...

Additional file 2: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Movies S2. Spawning behavior of the oral fertilization cichlid Ophtalmotilapia ventralis. (MOV 1450 ...

Additional file 7: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S2. Likelihood ratio test for dominance of fertilization types in nodes. (XLSX 8 kb

Additional file 10: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Table S3. Results from phylogenetic generalized least squares (PGLS) analyses of semen diffusion and...

Additional file 5: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Masaya Morita (3243033)
Stanley Ugwu (6109649)
Masanori Kohda (575198)

December 2018

Figure S1. Distinct fertilization types in Lake Tanganyikan cichlids and ancestral reconstruction of...

Additional file 11: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Abstract

Extracted data

Additional file 11: of Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Abstract

Extracted data

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