Impact of AID on target gene expression.

(A) Differential target gene mRNA expression of 6 non-AID expressors transfected with pGFP or pAID-GFP are shown. AICDA served as an internal control. Transcript levels were normalized to GAPDH and the relative fold changes are shown (2 -ΔΔCT method). The dashed lines show 3-fold increase or decrease in expression. M = IGHV mutated; UM = IGHV unmutated. From the 45 target genes, only the 26 genes with transcripts detectable at least in one sample are given on the x-axis. Samples without detectable expression of the respective gene are not shown. (B) Target gene mRNA levels of CLL cells from 32 CLL patients (AID neg = non AID-expressors; AID pos = AID expressors) detected by realtime RT-PCR are shown relative to GAPDH (2 -ΔCT method). The dashed line represents the detection limit (n.d. = not detected). From the 45 target genes, only 33 genes showed detectable expression and are listed on the x-axis.