Relationship between the sTLR-2 and the paired SEC associated TLR-2 transcript.

Unstimulated whole saliva (UWS) was collected from 10 individuals with normal oral health, 20 patients with gingivitis and 20 patients with chronic periodontitis. UWS samples were processed to separate clarified and epithelial cell rich fraction as described in materials and methods. The concentration of sTLR-2 was measured by ELISA. The SEC associated TLR-2 mRNA, the epithelial differentiation associated gene SPRR2a and the house keeping gene GAPDH was determined by quantitative PCR. Relative quantities of TLR-2 mRNA with respect to SPRR2a mRNA were determined using the 2-ΔCt method. (A) Shows no significant difference in the TLR-2 mRNA between the three groups, (B) shows gel electrophoresis of the PCR products, (C) shown a moderate inverse correlation between sTLR-2 and SEC TLR-2 mRNA in the gingivitis cohort and (D) shows a mild direct correlation between the sTLR-2 and SEC TLR-2 mRNA in the periodontitis cohort. Cohorts are labelled as N: normal, Gin: gingivitis and CP: chronic periodontitis.