Comparison of reactivity from ELISA against Peptides 1–6, Mix I, II, III, IV and SLiA.

ELISA was performed in different groups of individuals. Groups negative for VL (white circle): control group (CT, n = 70), Chagas’ disease (CD, n = 68). Groups positive for VL (black circle): visceral leishmaniasis (VL, n = 70). The anti-peptides 1–6, Mix I, II, III, IV and SLiA reactivity between groups were compared by Kruskal-Wallis followed by Dunn’s post-test for multi-group comparisons. The differences were considered statistically significant at ***p<0.001, highlighted by connecting lines. Cut-offs were determined by ROC curves.