Immunoblot analysis of Δ<i>espL</i> mutant.
- Claudia Sala (89990)
- Nina T. Odermatt (6107939)
- Paloma Soler-Arnedo (6107942)
- Muhammet F. Gülen (6107945)
- Sofia von Schultz (6107948)
- Andrej Benjak (53929)
- Stewart T. Cole (142597)
Publication date
January 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A) Total cell lysates prepared from the indicated bacterial strains were analyzed by immunoblot. Membranes were probed for RpoB, which represented the loading control, EspB, Antigen 85 (Ag85), EsxB, GroEL2, EspA, EspD and EsxA. B) Culture filtrates were analyzed as described for the total cell lysates. The experiment was repeated two times. One representative image is shown.</p
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