Gel mobility shift assays demonstrating effects of RNA structure on PPR10 binding.

The RNAs (5 pM) are diagrammed in Fig 1A. PPR10 was used at concentrations of 32 nM, 8 nM, 2 nM, and six additional 2-fold dilutions. Data for replicate assays (n = 2) are shown as separate points connected by a vertical line. (A) Summary of binding data for reactions incubated for 30 minutes. Representative gels are shown in S1B Fig. (B) Comparison of results for binding reactions incubated for 30 minutes and 2 hours.