<i>E</i>. <i>coli</i> speed in buffer and mucus after dosing to buffer or fed state medium.

Distribution of E. coli speeds in A) MB or B) mucus, after dosing in MB (black) or fed state medium (grey). Dashed lines represent individual experiments, and solid lines represent the overall average. C) Average speed of E. coli in MB or mucus after dosing in MB or fed state medium. Data represent mean ± standard error of three independent experiments, with n ≥100 microbes for each experiment, and significance was observed between all groups (*p < 0.05).