CSD suppresses both AngII-induced hypermigration of bone marrow cells (BMC) and AngII-induced vascular leakage of IgG into heart and kidney tissues.

Mice were infused with AngII or saline for 2 wk and received daily i.p. injections of CSD or vehicle. (A) Migration experiments were performed as described in the Methods using isolated BMC. Cells that migrated towards SDF-1 were counted in six high power fields per filter. Graphs show quantitation of migrated cells using three independent mice for each group. Statistically significant changes are shown as **p B) RIPA extracts of LV and kidney tissue were used in Western blotting experiments. HRP-conjugated anti-mouse secondary antibody with no primary was used to detect mouse IgG. (Left) Typical Western blot results from LV and kidney tissue showing IgGH (IgG heavy chain) and IgGL (IgG light chain). (Right) Quantification of IgGH and IgGL (n = 4). Statistically significant changes are shown as **p < 0.01, ***p < 0.001 for Sham+Veh vs AngII+Veh and ^^p < 0.01, ^^^p < 0.001 for AngII+Veh vs AngII+CSD.