Leakage of molecules through the <i>tyn</i> mutant body surface.

(A) Eosin Y penetrated the body of tyn1, y2, cho2, v1 larvae but not of y2, cho2, v1. Scale bar: 500 μm. (B) DAPI staining was observed in neither the epidermis nor trachea of the control larvae (+/+; Ubi-GAP-43GFP, His2AV-mRFP), while some of the epidermal and tracheal cells were strongly labeled with DAPI in the mutant larvae (tyn1, y2, cho2, v/Y; Ubi-GAP-43GFP, His2AV-mRFP). His2AV-mRFP (magenta) was expressed to visualize nuclei in all the cells. Bright-field images show that the trachea was gas-filled in the control but not in the mutant. Scale bar: 10 μm.